FIGURE 5.

Endorepellin activates the autophagic pathway via AMPKα phosphorylation. A, representative immunoblots of P-AMPKα (Thr¹⁷²) in response to endorepellin (200 nm) at different time points. B, quantification of P-AMPKα:AMPKα as shown in A. One-way analysis of variance was used for statistical analysis. C, representative immunoblots of P-AMPKα, total AMPKα, Beclin 1, and LC3-II following incubation with endorepellin (200 nm, 6 h), compound C (30 μm, 30 min pretreatment + 6 h), or endorepellin and compound C. D, quantification of the P-AMPKα:AMPKα ratio shown in C. E and F, quantification of Beclin 1 and LC3-II as shown in C after normalization to GAPDH. Quantification was performed using one technical replicate each from three biological replicates obtained from three independent experiments. D–F, *, p < 0.05, Student's t test.