FIGURE 8.

Depletion of TTF-1 promotes adipogenic differentiation of PPFP thyroid cells. A, PCCL3-EV and PCCL3-PPFP thyroid cells expressing either shControl or shTTF-1 were treated with DMSO or pioglitazone for 9 days followed by ORO staining of lipid droplets. B, quantification of ORO staining in A. C, relative mRNA expression of adipocyte genes at the conditions described in A was determined by RT-qPCR. D, upper panel, immunoblots for Fabp4, Plin2, and TTF-1 proteins were performed at the end of the differentiation described in A, and β-actin was used as a loading control; Lower panel, RT-qPCR was performed for mouse TTF-1 mRNA expression at the conditions indicated. A–D, data are representative of three independent experiments. Each qPCR analysis was performed in triplicate, and data were normalized to the expression of Pgk1. Statistical significance was evaluated with Student's t test, or ANOVA followed by Scheffe's test, *, p < 0.05 and **, p < 0.01