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. 2016 Jul 19;291(37):19274–19286. doi: 10.1074/jbc.M116.740324

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Stable overexpression of TTF-1 inhibits adipogenic differentiation of PPFP thyroid cells. A, immunoblots against TTF-1 or HA tag (to specifically detect exogenous HA-TTF-1) were performed following the stable overexpression of pMSCV-EV or pMSCV-HA-TTF-1 in PCCL3-PPFP rat thyroid cells. B, ORO staining of lipid droplets in pMSCV-EV or pMSCV-HA-TTF-1 expressing PCCL3-PPFP thyroid cells after 9 days of treatment with DMSO or pioglitazone. C, quantification of ORO staining in B. D, relative mRNA expression of adipocyte genes at the conditions described in B, determined by RT-qPCR. Each qPCR analysis was performed in triplicate, and data were normalized to the expression of Pgk1. E, immunoblots against Fabp4, TTF-1, or HA tag were performed at the end of the 9-day proadipogenic differentiation described above. A–D, data are representative of three independent experiments. C and D, statistical significance was evaluated with Student's t test, *, p < 0.05 and **, p < 0.01.