FIGURE 4.

Inhibition of mTORC1 inhibited the ability of TGF-β1 to increase DNMT3a. A, CCL210 cells were treated with TGF-β1 (2 ng/ml) in the presence or absence of rapamycin (1 μm), an mTORC1 inhibitor, and DNMT3a protein was assayed by immunoblot. The mean densitometric values of three independent experiments are shown below a representative immunoblot. *, p < 0.05. B, CCL210 cells were treated with either control siRNA or siRNA targeting Raptor. The cells were then treated in the presence or absence of TGF-β1 (2 ng/ml), and expression of DNMT3a and Raptor protein was assayed by immunoblot. Mean densitometric values of DNMT3a protein from four independent experiments are shown below a representative immunoblot. *, p < 0.05.