FIGURE 2.

KLF13 mRNA and protein levels are regulated by glucocorticoids in the heart and primary adult cardiomyocytes. Adrenalectomized (ADX) C57BL/6 male mice were injected with vehicle (Veh) or 1 mg/kg of Dex. RNA was isolated at 6 and 24 h after the treatment. Hearts were harvested for immunofluorescence at 24 h following Dex treatment. A, KLF13 mRNA levels in Veh (white bars)- and Dex (black bars)-treated mice. B, representative immunofluorescence staining of heart sections from control and cardioGRKO mice with anti-troponin I (green) and anti-KLF13 (red) antibodies. DAPI is shown in blue. Primary cardiomyocytes were treated with vehicle or Dex for 6 h. C, KLF13 gene expression levels in vehicle (white bars)- and Dex (black bars)-treated cardiomyocytes. D, representative immunofluorescence staining of Veh- and Dex-treated cardiomyocytes. Troponin I (red), KLF13 (green), and DAPI (blue nuclear staining) are indicated. Data represent mean ± S.E. (n = 3–5 mice per group). *, p < 0.05.