FIGURE 4.

KLF13 is a direct target of the glucocorticoid receptor in HL-1 mouse adult cardiomyocytes. A, HL-1 cells were treated with vehicle (control, white bars), 100 nm Dex (black bars), 1 μm RU486 (striped bars), or both 1 μm RU486 and 100 nm Dex (cross-hatched bars). KLF13 mRNA were measured by RT-PCR at the indicated times. B, HL-1 cells transfected with NTC siRNA or GR siRNA (−GR) were treated with 100 nm Dex for 3 h. mRNA levels were measured by RT-PCR. C, HL-1 cells were pretreated for 1–6 h with vehicle (control) or 10 μg/ml of cycloheximide (cyclo) then cells were exposed to 100 nm Dex for 3 h. KLF13 mRNA levels were assessed by RT-PCR. D, nascent RNA was measured by RT-PCR after treating the cells for 3 h with 100 nm Dex or vehicle (control). Data represent mean ± S.E. from three or four independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.