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. 2016 Jul 20;291(37):19387–19400. doi: 10.1074/jbc.M116.736074

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Inactive CdiA-CT^E479 and CdiA-CT^1026b toxin domains bind to endogenous tRNA. A, agarose gel analysis of catalytically inactive CdiA-CT^E479 and CdiA-CT^1026b toxins purified under non-denaturing conditions. Control RNA is from yeast (Sigma). B, size-exclusion chromatography of the purified tRNA/CdiA-CT(D214A)^1026b complex. C, size-exclusion chromatography of the purified tRNA/CdiA-CT(D243A)^E479 complex. Chromatography migration standards are as follows: bovine thyroglobulin (670 kDa), bovine γ-globulin (158 kDa), chicken ovalbumin (44 kDa), horse myoglobin (17 kDa), and vitamin B₁₂ (1.3 kDa). Each experiment was carried out in triplicate with similar results. Representative data are shown for each experiment.