TABLE 5.
Data collection and scattering-derived parameters for tRNA·CdiA-CT complexes
| Data collection parameters | tRNA/CdiA-CT1026b | tRNA/CdiA-CTE479 |
| Beam line | ALS 12.3.1 | ALS 12.3.1 |
| Wavelength (Å) | 1.0 | 1.0 |
| q range (Å−1) | 0.012–0.324 | 0.012–0.324 |
| Exposure time (seconds) | 0.5, 1, 2, 4 | 0.5, 1, 2, 4 |
| Concentration range (mg ml−1) | 0.5–5 | 2–10 |
| Temperature (K) | 283 | 283 |
| Structural parametersa | ||
| I(0) (cm−1) (from P(r)) | 248.1 ± 13.2 | 595.9 ± 13.4 |
| Rg (Å) (from P(r)) | 27.3 ± 1.3 | 46.9 ± 3.4 |
| I(0) (cm−1) (from Guinier) | 246.8 ± 14.9 | 590.6 ± 20.8 |
| Rg (Å) (from Guinier) | 27.1 ± 1.7 | 46.1 ± 4.0 |
| Dmax (Å)b | 91 | 190 |
| Porod volume estimate (Å3) | 43,041 ± 2,353 | 256,350 ± 2,517 |
| Dry volume calculated from Crysol14 (Å3)c | 45,510 | 43,112 (176,000)d |
| Molecular mass determinationa | ||
| Molecular mass (from Primus) (Da) | 41,539 ± 1,675 | 16,5473 ± 6,083 |
| Calculated monomeric mass from sequence (Da)c | 43,215 | 41,827 (167,308) |
a Data are reported for 5 mg ml−1 measurements.
b Dmax is a model parameter in the P(r) calculation.
c Values for tetramer are shown in parentheses.
d The estimated volume of the tRNA·CdiA-CTE479 complex is greater than the calculated volume from sequence due to the void volume assumed by the tetramer.