FIGURE 3.

Knockdown of CAT7 causes MNX1 overexpression and a loss of PcG-binding at the MNX1 promoter. A, MNX1 and CAT7 expression during posteriorized neuronal differentiation. B, RNAi of CAT7 or scrambled control (Scr) causes up-regulation of MNX1/ISl1 and down-regulation of IRX3 in posteriorized motor neurons, motor neuron precursors, and HeLa cells. C and D, several siRNAs or ASOs show selective knockdown of CAT7, and are accompanied by upregulation of MNX1 in neuronal precursors. E and F, ChIP-qPCR after RNAi of CAT7 yields loss of PRC1, PRC2, and H3K27me3 components at the MNX1 promoter in HeLa cells (C) and posteriorized neural culture (D), but no effect is seen at a control HOXD11 region (p-values from Student's t test normalized such that the IP performed in control siRNA treated cells = 100%). n = 3, error = S.D. from biological replicates. *, p < 0.05; ND, no data (not expressed).