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GSKIP recruits GSK3β from Axin. HEK293 cells were treated with siRNA to knock down the expression of GSKIP (siGSKIP) or with control non-targeting siRNA (siNT). GSK3β was immunoprecipitated, and GSK3β and co-immunoprecipitated Axin1 were detected by Western blotting (WB). Signals were semi-quantitatively analyzed by densitometry, and the ratio of precipitated Axin1 to GSK3β was calculated; n = 4, mean ± S.E., ANOVA, *, p < 0.05.
