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. 2016 Aug 2;291(37):19618–19630. doi: 10.1074/jbc.M116.738047

FIGURE 7.

FIGURE 7.

GSKIP, Axin1, Axin2, and AKAP220 compete for GSK3β binding. A, immunoprecipitation of YFP-GSK3β from HEK293 cells and detection of the indicated proteins by Western blotting (WB). The semi-quantitative analysis of co-immunoprecipitated HA-AKAP220 is depicted as the ratio of precipitated HA-AKAP220 to HA-AKAP220 in the input; data are normalized to cells expressing YFP-GSK3β and HA-AKAP220; n = 6, mean ± S.E., ANOVA, *, p < 0.05. B, co-immunoprecipitation of YFP-GSK3β and the indicated proteins from HEK293 cells. The proteins were detected by Western blotting. Semi-quantitative densitometric analysis of the signals is depicted as the ratio of precipitated GSKIP to precipitated GSK3β; n = 5, mean ± S.E., ANOVA, **, p < 0.01.