FIGURE 8.

GSKIP but not AKAP220 elevates the level of β-catenin in the cytosol in response to a Wnt signal. HEK293 cells expressing the wild type GSKIP or AKAP220 or an empty vector control were stimulated with control or Wnt3a-enriched medium, cytosolic fractions were prepared, and the indicated proteins were detected by Western blotting. The signals were semi-quantitatively analyzed by densitometry and depicted as ratios; n = 7, mean ± S.E., ANOVA, *, p < 0.05, ***, p < 0.001. pS675, Ser(P)-675.