FIGURE 1.

Bongkrekic acid and carboxyatractyloside decrease ATP turnover-linked respiration in rat hepatocytes but not in cancer cells. A, rat hepatocytes were maintained in Krebs-Ringer-HEPES buffer as described under “Experimental Procedures.” Respiratory rates were measured before and after the addition of ureagenic substrates (3 mm NH₄Cl, 5 mm ornithine, and 5 mm lactate). BA (10 μm), CAT (10 μm), OLIG (10 μg/ml), or vehicle (VEH) was added after ureagenic substrates. B and C, cancer cells were incubated in MHBSS. After measurement of baseline respiration, BA, CAT, OLIG, or VEH was added to A549 cells (B) and HepG2 cells (C).