FIGURE 3.

Bongkrekic acid, carboxyatractyloside, and oligomycin collapse mitochondrial membrane potential in hepatocytes after respiratory inhibition. A–C, rat hepatocytes in Krebs-Ringer-HEPES buffer were loaded with TMRM, as described under “Experimental Procedures.” After collection of baseline images, MYX (10 μm) was added, and another image was collected after 30 min. OLIG, BA, or CAT was then added, and images were collected after additional 30 min. Image intensity is pseudocolored according to the reference bar. D, average changes of fluorescence quantified as described under “Experimental Procedures” after various treatments are plotted. *, p < 0.05 versus baseline; †, p < 0.05 versus MYX. a.u., arbitrary units.