FIGURE 7.

miR-33 augments LPS proinflammatory cytokine induction via ABCA1 and ABCG1. A, PEMs from WT, Abca1^−/−, and Abcg1^−/− mice were transfected with negative control or miR-33 mimic as indicated and then exposed to LPS (100 ng/ml). TNFα and IL-6 protein in the cell medium were determined by ELISA. B, PEMs were transfected with control or miR-33 mimic (40 nm for 24 h), exposed to vehicle or TO901317 (10 μm for 12 h), and stimulated with LPS (100 ng/ml), and then TNFα and IL-6 in the cell medium were determined by ELISA. C, the indicated targets were probed by immunoblotting in cells treated as in B with miR-33 mimic/control and TO901317/vehicle. Data are the mean ± S.E. (error bars) of a representative experiment of at least three independent experiments. *, p < 0.05; **, p < 0.01.