TABLE 3.

G protein α subunit α1 helix interface energetic predictions

Entity	Amino acid	Ins4A-GTPγS		WT GTPγS		Ins4Aβ1γ1		WT β1γ2
		Energy	Z score	Energy	Z score	Energy	Z score	Energy	Z score
		REU		REU		REU		REU
β1	Leu38	1.1	75	1.2	281	1.4	53	2.0	17
P-loop	Gly40	0.8	103
α1	Lys46	1.3	53	1.1	89	1.9	6	1.9	35
α1	Ser47	0.7	61
α1	Thr48	0.9	52	0.8	154	0.7	27	0.9	27
α1	Ile49	1.0	38	1.0	179	1.1	192	1.0	10
α1	Val50			0.5	235	0.9	118
α1	Gln52	1.2	25	1.5	34	1.7	266	1.6	25
α1	Met53	0.5	6	1.4	462	1.0	116	1.4	14
α1	Lys54	1.8	122	2.3	101	2.2	441	2.5	24
α1	Ile55	1.4	170	1.0	263	1.0	158	1.0	10
α1	Ile56	1.0	85	1.1	57			1.1	35
α1	His57	1.3	28	1.6	133	1.5	127	1.7	34
Linker 1	Gly60			0.7	71	0.9	339	0.9	67
Linker 1	Tyr61	1.0	125	1.5	70	0.8	208	1.0	9
αA	Glu65	0.8	104	0.5	9	0.8	384	0.8	52
αF	Leu175	0.9	51	1.0	279	1.0	104	1.0	23
β2	Phe189	1.0	13	1.4	114	1.5	229	1.4	28
β2	Phe191
β3	Met198					0.5	73	0.5	6
β3	Asp200	0.8	84			1.0	3	1.0	24
β6-α5	Ala326	1.3	53	1.3	88	1.3	124	1.6	38
β6-α5	Thr329	0.5	21	0.8	100	0.8	346	0.8	16
α5	Val332	0.6	11	0.7	189	0.7	397	0.8	35
α5	Phe336			0.9	736			0.8	24