FIGURE 2.

Phosphorylation of ERK1/2 on Thr²⁰²/Tyr²⁰⁴, but not of MEK on Ser²¹⁷/Ser²²¹, is dependent on PKA activity. GCs were transduced overnight with Ad-GFP or Ad-PKI, a PKA inhibitor. Cells were then treated without (veh) or with FSH for 30 min. Samples were collected as described in Fig. 1A. The results are representative of four independent experiments.