TABLE 1.
Fractional RetGC1 activity in the presence of 0.1 μm RD3
The activity was assayed as described under “Experimental Procedures.” The fractional activity was defined as ARD3/A0, where A0 is the RetGC1 activity in the absence of RD3 in the standard assay and ARD3 is the RetGC1 activity in the presence of 0.1 μm RD3.
| Fragment | Mutation | Fractional RetGC1 activity |
|---|---|---|
| 21PAEM24 | APME | 0.21 ± 0.02 |
| 27ETLM30 | QMVT | 0.51 ± 0.02 |
| 35GQMR38 | ERLG | 0.19 ± 0.01 |
| 41ERQQ44 | KREQ | 0.22 ± 0.01 |
| 50AVRK53 | KAIE | 0.13 ± 0.01 |
| 55CTGVD59 | GNCDV | 0.34 ± 0.02 |
| 77ERLQ80 | QLRE | 0.12 ± 0.01 |
| 82EDVC85 | QVDS | 0.39 ± 0.01 |
| 87KIHP90 | PHRL | 0.86 ± 0.01a |
| 93CGPAI97 | PALSL | 0.93 ± 0.02a |
| 99RFRQ102 | FRQR | 0.87 ± 0.08a |
| 107QEPEV111 | PVQEE | 0.40 ± 0.01 |
| 112QEV114 | EVQ | 0.35 ± 0.01 |
| 119RSVL122 | LAER | 0.80 ± 0.01a |
| Met129 | D | 0.11 ± 0.00 |
| 132EEE134 | KKK | 0.12 ± 0.01 |
| 137KLTRQ141 | QRKTL | 0.09 ± 0.01 |
| 147RGSL150 | GRLS | 0.10 ± 0.01 |
| 154KTRAR158 | ENSES | 0.08 ± 0.01 |
| 164SDIRT168 | RSDTI | 0.10 ± 0.01 |
| 170EDVERD175 | KKAKQR | 0.05 ± 0.03 |
| 178PPP180 | AAA | 0.10 ± 0.03 |
| 186SMP188 | PRS | 0.13 ± 0.00 |
| Wild type | 0.08 ± 0.01 |
a RD3 mutants for which ARD3 ≥0.8 A0 in the standard assay conditions.