Figure 5. Inflammation does not precede the onset of the phenotypic modifications observed in Bmpr1a^ΔMES mice.

(a) Staining against immune cells was performed on the corpus glands of 30-day and 90-day-old mutant and control mice. Staining against CD3 in 30-day-old mice showed no presence of T cells in the mucosa of controls while minimal staining is seen in mutant mice. Cross reactivity (green labelling above the mucosa) with food present in the control stomach lumen is observed. (b) Staining against CD3 in 90-day-old mice revealed a prominent presence of T cells in the mucosa of Bmpr1a^ΔMES mice whereas T cell still remained absent in control mice. Evans Blue served as counterstain (red staining). (c) Staining against F4/80 in 30-day-old mice showed no presence of macrophages in the mucosa of control mice where minimal staining is observed in mutants. (d) Staining against F4/80 in 90-day-old mice revealed a marked of presence of macrophages in stromal tissue of Bmpr1a^ΔMES mice while macrophage staining was not observed in control mice. Scale bar: 50 μm.