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. 2016 Sep 9;6:33168. doi: 10.1038/srep33168

Figure 8. Egg surface sterilization for elimination of the symbiotic bacterium.

Figure 8

(a) Surface sterilization of G. lineatum eggs with hypochlorite sodium to eliminate bacterial symbionts. Bacterial population declined after sterilization of eggs (b) qPCR analysis of DNA extracted from untreated eggs (control) and surface-sterilized eggs of G. lineatum. Bacterial symbiont population dramatically declined after sterilization of eggs (c) qPCR analysis for bacterial symbiont intensity in first nymphs of G. lineatum hatched from control and surface-sterilized eggs with hypochlorite sodium for 15 s. Egg sterilization decreased bacterial symbiont intensity in treated nymphs in comparison with the controls.