Fig. 1.

Production and characterization of recombinant CSFV E2 protein. a SDS-PAGE analysis. Lane 1, E2 protein (1 μg) treated with Laemmli sample buffer with addition of reducing reagent β-mercaptoethanol (β-ME); Lane 2, E2 protein (1 μg) treated with Laemmli sample buffer without β-ME. b Western blot of purified E2 protein. Lane 1, E2 protein (1 μg) treated with β-ME; Lane 2, E2 protein (50 ng) purified and stored under non-reducing conditions. E2-specific Mab WH211 was used for the western blot