Table 2.
CSFV were cleared from the nasal cavity in pigs vaccinated with E2 subunit vaccine 15 days post challenge
| Nasal swab | 3 DPC | 6 DPC | 9 DPC | 12 DPC | 15 DPC | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Treatment | Pig # | Ct value | TCID50 | Ct value | TCID50 | Ct value | TCID50 | Ct value | TCID50 | Ct value | TCID50 |
| (−/−) | 51 | ND | ND | ND | ND | ND | ND | ND | ND | (−) | (−) |
| (−/−) | 52 | ND | ND | ND | ND | ND | ND | (−) | (−) | (−) | (−) |
| (−/−) | 53 | (−) | (−) | (−) | (−) | (−) | (−) | (−) | (−) | (−) | (−) |
| (−/−) | 54 | (−) | (−) | (−) | (−) | 39 | 37 | (−) | (−) | (−) | (−) |
| (−/−) | 55 | (−) | (−) | 34 | 184 | 34 | 560 | (−) | (−) | (−) | (−) |
| (−/+) | 56 | (−) | (−) | 35 | 95 | 33 | 1521 | 28 | 19,033 | 33 | 2867 |
| (−/+) | 57 | (−) | (−) | 32 | 876 | 24 | 300,220 | 20 | 1,882,476 | 22 | 904,988 |
| (−/+) | 58 | 37 | 31 | 34 | 293 | 24 | 411,326 | 21 | 1,438,113 | 21 | 1,502,256 |
| (−/+) | 59 | (−) | (−) | 32 | 1065 | 21 | 3,182,081 | pig died | |||
| (−/+) | 60 | 38 | 19 | 34 | 261 | 25 | 293,326 | 21 | 1,451,296 | 22 | 745,304 |
| One-dose | 61 | (−) | (−) | 35 | 124 | 36 | 166 | 36 | 106 | (−) | (−) |
| One-dose | 62 | (−) | (−) | (−) | (−) | 38 | 52 | 37 | 42 | 36 | 109 |
| One-dose | 63 | (−) | (−) | 37 | 35 | 37 | 83 | 33 | 495 | (−) | (−) |
| One-dose | 64 | (−) | (−) | 34 | 224 | (−) | (−) | 37 | 68 | (−) | (−) |
| One-dose | 65 | (−) | (−) | 36 | 68 | 37 | 78 | 34 | 301 | (−) | (−) |
| Two-dose | 66 | (−) | (−) | 37 | 38 | 39 | 28 | 35 | 145 | (−) | (−) |
| Two-dose | 67 | (−) | (−) | 36 | 60 | 38 | 67 | 35 | 221 | (−) | (−) |
| Two-dose | 68 | (−) | (−) | (−) | (−) | 34 | 2398 | 34 | 283 | (−) | (−) |
| Two-dose | 69 | (−) | (−) | 36 | 56 | 37 | 79 | 34 | 269 | 37 | 42 |
| Two-dose | 70 | (−) | (−) | 36 | 58 | (−) | (−) | 34 | 330 | (−) | (−) |
ND not done; (−): Undetectable; Pigs were challenged with CSFV 35 days post first vaccination. CSFV RNA in the blood was measured by real-time RT-PCR as described in Methods section
(−/−): Control pigs without CSFV challenge; (−/+): Control pigs challenged with CSFV
One-dose: Pigs vaccinated with one dose KNB-E2 and then challenged with CSFV
Two-dose: Pigs vaccinated with KNB-E2 twice and then challenged with CSFV