Table 1.
Sequences of oligonucleotide primers and details of polymerase chain reactions
| mRNA | Primers (5′→3′) | Bases in base pairs | Product size | Cycles profile | Cycles number |
|---|---|---|---|---|---|
| Denaturation/annealing/extension (temperature and time in seconds) | |||||
| β-actin | F: AGGGAAATCGTGCGTGACAT | 694–713 | 150 | 95/95/60 °C (20/3/30 s) |
40 |
| R: GAACCGCTCATTGCCGATAG | 824–843 | ||||
| Thy-1 | F: ACCAAGGATGAGGGCGACTA | 380–399 | 120 | 95/95/60 °C (20/3/30 s) |
40 |
| R: CAGGCTTATGCCACCACACTT | 479–499 | ||||
| MMP-9 | F: TGCGCTGGGCTTAGATCATT | 1218–1237 | 105 | 95/95/60 °C (20/3/30) |
40 |
| R: TGGATGCCTTTTATGTCGTCTTC | 1300–1322 |
F forward; R reverse