Table 3.

Choline acetyltransferase (ChAT) immunolabelling

Group	1	2	3	4
Cholinergic amacrines (number/field) on day 1	34.33 ± 6.59	12.67 ± 2.80*	28.33 ± 2.16†	31.67 ± 3.88†
Cholinergic amacrines (number/field) on day 7	36.83 ± 6.68	13.17 ± 4.07*	23.50 ± 3.45†	28.50 ± 4.59†

In comparison of the control retina, 1 or 7 days following sham procedure (Sham; Group 1), after retinal ischemia plus 1 or 7 days of reperfusion and pretreatment with vehicle (I/R + vehicle; Group 2), the number of CHAT-immunolabeling amacrine cells per field was significantly decreased (* P < 0.05) in the inner nuclear layer and the ganglion cell layer. In contrast, this significant decrease was dose-dependently (with a less effect at 2.8 g/kg/day, I/R + CJDHW2.8, Group 3) and significantly (^† P < 0.05; Group 3; I/R + CJDHW4.2, Group 4) inhibited by pretreatment with CJDHW. The results are the mean ± SD (n = 6)