Table 2.
Primary antibodies used for immunoblotting in this study
| Antibody (catalogue no.) | Species | Dilution | Company |
|---|---|---|---|
| β-Actin (#2228) | mouse mAb | 1:5000 | Sigma |
| CDK5 (#2506) | rabbit pAb | 1:1000 | CST |
| CRMP2, clone C4G (#11096) | mouse mAb | 1:1000 | IBL |
| CRMP2, pSer522 (#CP2191) | rabbit pAb | 1:1000 | ECM |
| CRMP2, pThr514 (#9397) | rabbit pAb | 1:1000 | CST |
| CRMP2, phosphorylated clone 3 F4 (#29060) | mouse mAb | 1:200 | IBL |
| GAPDH (#G8795) | mouse mAb | 1:10000 | Sigma |
| GSK3β, clone 3D10 (#9832) | mouse mAb | 1:500 | CST |
| GSK3β, pSer9 (#5558) | rabbit mAb | 1:2000 | CST |
| GSK3β, pTyr216 (#ab75745) | rabbit pAb | 1:2000 | Abcam |
| p35 (#sc-820)a | rabbit pAb | 1:200 | SC |
| PP2A, C subunit (#2038) | rabbit pAb | 1:1000 | CST |
| Synaptophysin (#MAB368) | mouse mAb | 1:2500 | MM |
| α-Synuclein (#sc-7011-R) | rabbit pAb | 1:500 | SC |
| β-III-Tubulin, clone TU-20 (#ab7751) | mouse mAb | 1:1000 | Abcam |
mAB monoclonal antibodies, pAB polyclonal antibodies. Company abbreviations: Abcam Abcam plc (UK), CST Cell Signaling Technology (USA), ECM ECM Biosciences (USA), IBL IBL-International (Germany), MM Merck-Millipore (Germany), SC Santa Cruz Biotechnology (USA), Sigma Sigma-Aldrich (USA)
aAlso recognizes p25