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. 2016 Sep 8;13(1):241. doi: 10.1186/s12974-016-0705-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Gly administration blocked T lymphocyte infiltration in the ischemia brain. Mononuclear leukocytes were extracted from ischemic brains 3 days after stroke, and the absolute numbers of lymphocyte subsets were analyzed by FACS. Contralateral hemisphere was collected as control. The drug was injected immediately and 3.5 h after suture removal. a Gating strategies for identifying CD4 and CD8 T cells. b Bar graphs for statistical results of T lymphocytes and T cell subsets. T cells, CD4, and CD8 cells were assessed. n = 7 to 12/each group. *P < 0.05 between the two indicated groups