Fig. 5.

Effect of Gly injection on T cell proliferation. a An ex vivo assay suggests that T cell proliferation was inhibited in splenocytes harvested from animals injected with Gly. The drug was injected twice in rats at an interval of 3.5 h. No stroke or sham surgery was performed. Splenocytes were harvested 5, 12, 24, 48, and 72 h after the last drug injection, cultured, and stimulated with ConA. Proliferation was assessed by measuring cell numbers taken by the [³H]-thymidine incorporation assay. ^### P < 0.001 vs naïve; ^@@@ P < 0.001 vs 24 h with ConA injection. b In vitro assay shows that Gly dose-dependently inhibited T cell proliferation stimulated by ConA. Splenocytes (2 × 10⁵) harvested from naïve rats were cultured and stimulated with ConA and [³H]-thymidine, and different dosages of Gly were added. T cell proliferation was then measured. CPM counts per minute, which is the counted radioactivity. **P < 0.01 between the two indicated groups