Fig. 8.

The effect of Gly on rhHMGB1-mediated T cell proliferation in cultured splenocytes. a rhHMGB1 protein promoted splenocyte proliferation in vitro. Splenocytes harvested from naïve rats were cultured, and rhHMGB1 was added in a series of concentrations. The cell numbers were detected using Cell Counting Kit-8. The results suggest that rhHMGB1 dose-dependently increased splenocyte proliferation. *P < 0.05, ***P < 0.001 vs 0 ng/ml of rhHMGB1, respectively. ^@@@ P < 0.001 between the two indicated groups. b The addition of Gly inhibited rhHMGB1-mediated T cell proliferation. Different concentrations of Gly were added to the cultured splenocytes with rhHMGB1 and ConA, and T cell proliferation ratios were measured. *P < 0.05, **P < 0.01, ***P < 0.001, respectively, between the two indicated groups. n = 5/group. OD optical density, CPM counts per minute