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. 2016 Sep 9;6:32932. doi: 10.1038/srep32932

Figure 1. Changes of germ cell-specific gene expression in MEFs in OCKS + chem + VA5 + Dnmt1-KD condition.

Figure 1

The expression of germ cell-specific genes was quantified by real-time PCR in MEFs after transfection of the expression vector encoding the Yamanaka factors (Oct4, c-Myc, Klf4, Sox2: OCKS) with or without addition of tranylcipromine, BIX-01294, DZNep (chem), VPA, ALK5i (VA5), and Dnmt1 Knocked-Down (KD) (OCKS + chem + VA5 + Dnmt1-KD, or OCKS + Ctrl siRNA) after 2, 3, 4 days in culture. The expression level of each gene in MEFs with OCKS + chem + VA5 + Dnmt1-KD was set as 1.0. Error bars: S.E. of three biological replicates, *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test).