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. 2016 Sep 9;6:32932. doi: 10.1038/srep32932

Figure 2. Yamanaka factors were not necessary for induction of germ cell-specific genes.

Figure 2

(a) Vasa and Dazl expression in the presence of OCKS or OS with chem + VA5 + Dnmt1-KD after 4 days in culture. (b) The expression of germ cell-specific genes with or without OCKS in chem + VA5 + Dnmt1-KD condition after 2 days in culture. The expression of germ cell-specific genes was quantified by real-time PCR. The expression level of each gene in MEFs with OCKS + chem + VA5 + Dnmt1-KD was set as 1.0. Error bars: S.E. of three biological replicates, *p < 0.05, n.s.: not significantly different (Student’s t-test).