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. 2016 Sep 9;6:32932. doi: 10.1038/srep32932

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) The DNA methylation status in the Dazl, Tex19.1, and Sycp1 promoter regions in MEFs 4 days after the OS + chem + VA5 + Dnmt1-KD treatment or in control MEFs. DNA methylation status was determined by bisulphite sequencing. The filled and open circles indicate methylated- and un-methylated CpGs, respectively. The data shown were combined from two independent experiments. The percentage of methylated-CpGs is shown. (b) ChIP analysis of the promoter regions of Dazl, Tex19.1, and Sycp1 for H3K4me3 and H3K27me3 in MEFs 4 days after the OS + chem + VA5 + Dnmt1-KD treatment or in control MEFs. ChIP using normal IgG was used as negative control. Levels of H3K4me3 or H3K27me3 were determined by real-time PCR, and the percentages of values relative to those for input chromatin are shown. Representative data from two independent experiments are shown. See also Supplementary Fig. S9.