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. 2016 Sep 9;6:32939. doi: 10.1038/srep32939

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Copyright © 2016, The Author(s)

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(a,b) qRT-PCR analysis of BmATG8 (a) and BmATG1 (b) in midgut cells after administration of 20E; (c,d) Western blot analysis of BmAtg8–PE (c) and acid phosphatase activity (d) in midgut of larvae injected with the hormone; (e) Western blot analysis of p-Bm4ebp1 in hormone-treated larvae. Values represent mean ± s.e.m. (**p < 0.01 using Student’s t-test).