Figure 4. Rapamycin fails to activate a full autophagic response.
(a) Western blot analysis of p-Bm4ebp1 in midgut of larvae injected with rapamycin; (b,c) qRT-PCR analysis of BmATG8 (b) and BmATG1 (c) expression after rapamycin treatment; (d,e) Western blot analysis of BmAtg8–PE (d) and acid phosphatase activity (e) after administration of rapamycin. Values represent mean ± s.e.m. (**p < 0.01 using Student’s t-test).