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. 2016 Sep 8;13:49. doi: 10.1186/s12989-016-0160-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Glutathione redox state in response to CuO NPs. A549 cells treated with CuO NPs at 0, 2.5, 5.0, 10, 20 and 40 μg/ml for 1, 3, 6, 12 and 24 h. Data is expressed as GSH/GSSG ratio, and each data point represents the mean ± SEM (n = 4). Statistical significance (determined by ANOVA with Tukey posthoc) is shown by * = p < 0.05, ** = p < 0.01, *** = p < 0.005, when compared to medium only treated cells