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. 2016 Sep 9;6:33149. doi: 10.1038/srep33149

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The sensorchip immobilized with the t8E4 antibody was incubated with five different concentrations (6.25, 12.5, 25, 50, and 100 nM) of tcK2SP(Xa) or tcK4SP(Xa) analytes. Actual binding curves (lavender) are overlaid with the fitting curves (thin black lines) used to derive the K_D values. Experiments were repeated three times and the K_D values are expressed as mean ± SD (n = 3) at the top.