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. 2016 Sep 8;12(1):195. doi: 10.1186/s12917-016-0811-8

Fig. 1.

Fig. 1

Serum IgG immune response in mice inoculated with T1 C. pseudotuberculosis strain, as evaluated by ELISA. Graph represents means of Optical Density (OD) values found for each group (n = 5 animals for group). Results are representative of the mean values obtained from two experiments. a. BALB/c mice were inoculated with increasing dosages: 5x105, 1x106, 5x106 and 1x107 CFU. Blood was collected 120 days after inoculation. Data were analyzed by ANOVA and Tukey post-hoc tests; *, †, ‡ and § indicate pairs with statistically significant differences. b. IgG subclass (IgG1, IgG2a, IgG2b and IgG3) production throughout the course of the experiment: control (before infection), 7, 30, 60, and 120 days after infection. Mice were inoculated with 107 CFU of T1 strain of C. pseudotuberculosis. Data were analyzed by ANOVA. *P < 0.05; ***P < 0.001