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. 2016 Jul-Aug;32(4):880–885. doi: 10.12669/pjms.324.9908

Table-I.

Primers, restriction enzymes and PCR digested fragments of IL-6-174G>C, -572G>C and -597G>A

IL-6 Primers(5’-3’) Restriction enzymes Digested fragments
-174G>C TGACTTCAGCTTTACTCTTTGT
CTGATTGGAAACCTTATTAAG
NlaIII GG: 164bp and 59bp
GC: 164bp, 111bp, 59bp and 53bp
CC: 111bp, 59bp and 53bp
-572G>C GAGACGCCTTGAAGTAACTG
AACCAAAGATGTTCTGAACTGA
BsrBI GG: 122bp and 60bp
GC: 182bp, 122bp and 60bp
-592G>A CTCCTCTAAGTGGGCTGAAG
CAAGCCTGGGATTATGAAGA
RsaI GG: 412bp
GA:412bp, 236bp and 176bp
AA: 236bp and 176bp