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. 2016 Sep 9;22(1):25. doi: 10.1186/s40409-016-0080-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Gene construction of the plasmid carrying HisrMlat1. a The nucleotide and amino acid sequence of HisrMlat1. b The nucleotide and amino acid sequence of HisrMlat1 after modification of the Arg N-terminal. c Scheme of the expression vector pQE30XaHisrMlat1. The His-tag is part of the 5′-nucleotide sequence of pQE30, in such a manner that the sequence to be expressed is the one shown, in which the cleavage site for the restriction enzyme is indicated (BamHI and PstI). d Amino acid sequence of HisrMlat1. The His-tag is in bold, the FXa cleavage site is in italics and the modification of the residue Lys is in bold