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. 2016 Sep 9;6:33037. doi: 10.1038/srep33037

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The SILEX plasmid encoding hHsp70 was designed with different copy numbers (low or high), nature of the promoter (T7 or T7lac), and strength of the ribosome binding site (RBS) (strong or weak). For each resulting SILEX system, PNP expression was analyzed by SDS-PAGE. The bands corresponding to hHsp70 and PNP are indicated on the gel. The molecular weight markers are indicated on the left of the gels in kDa.