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. 2016 Sep 9;6:33037. doi: 10.1038/srep33037

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The figure summarizes the autoinduction mechanism. (1, 2) During the first stage of growth, a small quantity of heterologous hHsp70 is produced (coded by the plasmid 1). (3) hHsp70 interacts with endogenous E. coli GAPDH. (4) Progressively, the increase of E. coli GAPDH induces metabolic changes that most likely drive the strong induction. In this step, the lactose in the growth medium is necessary to accumulate the inducer represented by a blue square. (5) The induction of plasmid 1 leads to amplification of the phenomenon. Finally, the induction of the expression of plasmid 2 (6, 7) lead to high production of the protein of interest (8).