Table 1. Modulation of the SILEX induction with lactose or glucose.
| LB (OD60 nm before-after first recombinant protein detection) | LB + 0.05% glucose | LB + 0.2% lactose | |
|---|---|---|---|
| MsrB | 0.9–1.2 ± 0.1 | 1.5–1.6 ± 0.1 | 0.7–1.0 ± 0.1 |
| Trx1 | 1.1–1.3 ± 0.1 | 1.6–1.8 ± 0.1 | 0.4–0.9 ± 0.1 |
| PNP | 1.0–1.1 ± 0.1 | 1.3–1.6 ± 0.1 | 0.6–1.0 ± 0.1 |
| T1R1 | 1.0–1.3 ± 0.1 | 1.2–1.6 ± 0.1 | 0.5–0.8 ± 0.1 |
| MCL | 0.9–1.1 ± 0.1 | 1.6–1.8 ± 0.1 | 0.7–1.2 ± 0.1 |
To measure induction, a culture sample were analyzed by SDS-PAGE and the OD60 nm was monitored every 15 minutes during log phase. For each recombinant protein, the OD60 nm corresponding to the measurement point before and after first detection on SDS-PAGE was indicated in the table.