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. 2016 Sep 9;6:32887. doi: 10.1038/srep32887

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HepG2 cells were treated with 0.1% DMSO or DAPA at various concentrations (0.8, 4, 20, 30 μM) for 24 hr. Quantitative RT-PCR and Western blot were performed for the measurement of mRNA and protein. (A,B) ZAG mRNA (A) and protein (B) expression in HepG2 cell lysate. (C) ZAG protein levels in HepG2 culture medium. (D,E) ADI mRNA (D) and protein (E) expression in HepG2 cell lysate. (F) ADI protein levels in HepG2 culture medium. (F,G) ADI mRNA (G) and protein (F) expression in 3T3-L1 adipocytes lysate. DAPA, dapaglifozin; ZAG, zinc-α₂-glycoprotein; ADI, adiponectin. The results represent three separate experiments performed in duplicates as means ± SEM. *P < 0.05, **P < 0.01 vs. controls.