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. 2016 Sep 9;7:1275. doi: 10.3389/fmicb.2016.01275

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Copyright © 2016 Devasundaram, Gopalan, Das and Raja.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) General gating strategy for T cell is given. After selecting the singlets from lymphocyte population, CD3⁺ cells were gated followed by CD4⁺ cells were gated and selected for memory phenotyping. (B) CD4⁺ cells were analyzed for the expression of CD62L and CD45RA surface markers to define the subtypes of memory cells. Antigen specific expansion of the central memory phenotype is evident in healthy household contacts (HHC) and representative flow diagram from HHC and pulmonary TB (PTB) is given. (C) Percentage of central memory cells specific to ESAT-6 (E6) and Lpd were shown as bar graphs representing mean with SEM. p-values were calculated using the Mann–Whitney U test and value <0.05 considered as significant.