Table 3.
Details of overexpressed and newly appearing proteins identified by mass spectrometry from M. tuberculosis laboratory strain S10 under hypoxia compared to aerated cultures.
| Spot no | Rv no | Protein Name | Predicted M.Wt (kDa) | Predicted pI | Coverage (%) | Peptide matched |
|---|---|---|---|---|---|---|
| S10D1 | Rv0440 | GroEl2 | 65 | 4.8 | 11% | TDDVAGDGTTTATVLAQALVR |
| S10D2 | Rv0350 | Probable chaperone protein DNAK | 75 | 4.9 | 23% | HMGSDWSIEIDGKK, GIPQIEVTFDIDANGIVHVTAK |
| S10D3 | Rv0462 | Dihydrolipoamide dehydrogenase (LpdC) | 50 | 5.8 | 25% | VAGVHFLMK, ATFCQPNVASFGLTEQQAR |
| S10D4 | Rv1240 | Probable malate dehydrogenase (MDH) | 48 | 4.9 | 16.7% | NAAEVVNDQAWIEDEFIPTVAK, SDLLEANGAIFTAQGK |
| S10D5 | Rv0351 | GrpE | 27 | 4.2 | 31% | PDGNSGEQVTVTDKRR, PVIGTVMRQGYQLGEQVLR |
| S10D6 | Rv3029c | Probable electron transfer flavo protein (β-subunit) | 31 | 4.8 | 4.90% | EAADAVLDEINER |
| S10D7 | Rv2032 | Conserved protein acg | 30 | 5.7 | 7% | IDVIADDMRPELAAASK |
| S10D8 | gi| 33742142 | Sequence 142 from patent US 6583266 (corresponding atpA) | 23 | 4.2 | 3.70% | VVNPLGQPIDGR, GTTIASVRR |
| S10D9 | Rv3841 | Possible bacterioferritin (BfrB) | 20 | 4.3 | 13.30% | VEIPGVDTVR, AGANLFELENFVAR |
| S10D10 | Rv3841 | Possible bacterioferritin (BfrB) | 20 | 4.6 | 42% | NHAMMLVQHLLDR, VEIPGVDTVR, EALALALDQER, VALMATLVR, AGANLFELENFVAR, EVDVAPAASGAPHAAGGRL |
| S10D11 | gi| 518086696 | Cyclase [M. tuberculosis] | 20 | 4.8 | 20.80% | AIADIEAYPQWISEYK, QSLSWTLESSSLLK |
| S10D12 | Rv3716c | Conserved protein | 15 | 4.4 | 19% | VVDPDDIETLQDLIVGAMR |
| S10D13 | Rv2031c | HspX | 16 | 4.7 | 29% | ATTLPVQRHPR, AELPGVDPDKDVDIMVR, SEFAYGSFVR |
| S10D14 | Rv1636 | Iron-regulated universal stress protein family protein TB15.3 | 14 | 5.8 | 37% | LIIASAYLPQHEDAR, DESYKVTGTAPIYEILHDAK, LLGSVPANVSRRAK |
| S10D15 | Rv3418c | 10 kDa chaperonin (GROES) | 12 | 4.5 | 15% | IPLDVAEGDTVIYSK |
| S10D16 | Rv3418c | 10 kDa chaperonin GROES | 12 | 4.5 | 27% | IPLDVAEGDTVIYSK, YNGEEYLILSAR |
| S10D17 | Rv2626c | Hypoxic response protein 1 (Hrp1) | 12 | 4.7 | 9% | DIMNAGVTCVGEHETLTAAAQYMR |
Mass spectrometry characterization of anaerobic proteins of S10 (designated as S10D). Underlined spot numbers indicate the newly appearing proteins during hypoxia. For few protein spots accession number was given since the gene number was not described in protein search tool used here.