Table 1.

Detection sensitivity of MS-UC-FRET, RQ-PCR and pyrosequencing for CDKN2A methylation (number of replicates with CDKN2A methylation detected).

Methylation%	0	0.01	0.05	0.1	1	5	10	20	50	100
	No. positive/No. tested
UC-MS-FRET	NEG	4/10	2/10	10/10	10/10	10/10	10/10	10/10	10/10	10/10
RQ-PCR	0/4	0/4	0/4	0/4	0/4	4/4	4/4	4/4	4/4	4/4
pyrosequencing	NEG	ND	ND	ND	ND	2/2	2/2	2/2	2/2	ND

Abbreviations: NEG: negative; ND: not done.