Fig 3. Effect of Foxs (Foxm1, Foxo6, Foxk2, and Foxs1) on the adipogenesis of brown preadipocytes.

(A) Expression of Foxm1, Foxo6, Foxk2, and Foxs1 in siRNA-transfected primary brown preadipocytes. Primary brown preadipocytes were transfected with siRNA for Foxm1, Foxo6, Foxk2, and Foxs1, and their expression was measured by qPCR (B) Oil red O staining of brown preadipocytes transfected with siRNAs for Foxm1, Foxo6, Foxk2, and Foxs1. Primary brown preadipocytes were transfected with siRNAs for Foxm1, Foxo6, Foxk2, and Foxs1, and were then differentiated in differentiation medium for 7 days. Lipid accumulation was assessed by Oil Red O staining. qPCR data are presented as means±SEM from three independent experiments. *P< 0.05 vs. scramble.