Fig 4. Enhanced cytotoxic effects of pterostilbene in precancerous cell lines, HBECR and HBECR/p53i.
(A) Western blotting analysis showing the presence and absence of p53 in HBECR and HBECR/p53i cell lines. (B) Cell viability of HBECR and HBECR/p53i cells (2×103) treated with different concentrations of pterostilbene ranging from 0 to 100 μM. (C) Cell proliferation ELISA assay with BrdU incorporation. (D) HBECR and HBECR/p53i cell lines were synchronized by double thymidine block. The HBECR cells were only partly synchronized. Cells were released into S phase along with treatment of pterostilbene at the final concentration of 3 μM. At the indicated time point from the thymidine block, cells were harvested and fixed for cell cycle analysis.