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. 2016 Sep 9;11(9):e0162733. doi: 10.1371/journal.pone.0162733

Fig 3. In vitro tryptic digestion of pentameric GLP-1 detected by Western blot.

Fig 3

The wt 5×GLP-1 and trp 5×GLP-1 purified from E. coli and L. paracasei BL23 (KKA394 and KKA405) were digested using the trypsin spin column for 7 min. The 250 ng of purified protein (P) and 250 ng of digested protein (D) were loaded in parallel on SDS-PAGE and detected by silver staining. The arrow indicates the size of the GLP-1 monomer (3.4 kDa).