Fig 4. Cdc6 depletion inhibits mitotic exit in synchronized cells.

HepG2 cells were synchronized by double thymidine block, and then released into fresh medium containing PTX (30 nM) or PTX (30nM) + NCTD (30 μM). For Cdc6 RNAi, the transfection was performed after first thymidine block. Cell cycle profiles were analyzed by FACS after released for indicative time. G2/M (left) and G1 (right) percentage are presented in below panel. Three independent experiments were performed.