Fig 7. Cdc6 depletion inhibits PTX-induced mitotic slippage in Hela cells.

(A) Hela cells were treated with PTX (30 nM) for 1 to 5 days, NCTD (30 μM) was added to replace PTX after 3 days PTX treatment. Cdc6 RNAi was transfected after PTX treatment for 3 days. Cells were stained with Giemsa dye and the percentage of slippage cells is calculated (right panel). (B) Cell viability after PTX treatment combined with or without NCTD (30 μM) for 7 days was valued by typan blue assay. All of the experiments were performed in three independent experiments, *P<0.05 as compared to PTX group.